T7701
Sigma
TargeTron® Vector pJIR750ai
Description
| General description | TargeTron® vector pJIR750ai is a 10,262 bp expression vector intended for use with the TargeTron Gene Knockout System, Catalog Number TA0100. This circularized vector can be used for targeted gene knockouts in gram-positive bacteria such as Clostridium perfringens type A.1 Expression of the group II intron RNA is under the control of the β-2 toxin gene promoter, cpb2.1 As supplied, the pJIR750ai vector is re-targeted to the α-toxin (plc) gene. α-toxin, a phospholipase C, is an extracellular toxin produced by all C. perfringens isolates.1 For validation in a specific C. perfringens strain, this vector can be used directly to knockout plc without any further modifications using the enclosed protocol. In order to re-target this vector to knockout other genes, the plc specific IBS-EBS fragment (350 bp) between the Hind III and BsrG I sites can be cut out and replaced with another gene specific fragment. For re-targeting protocols, refer to the TargeTron Gene Knockout System User Guide, Catalog Number TA0100, at sigma-aldrich.com. |
| Legal Information | TargeTron is a registered trademark of InGex, LLC |
Properties
| storage temp. | −20°C |
References
| reference | Chen, Y., et.al. Appl. Environ. Microbiol. 71, 7542-7547, (2005) Abstract |
| Yao, J., et.al. RNA 12, 1271-1281, (2006) Abstract | |
| Frazier , C., et al Appl. Environ. Microbiol. 69th ed.,, 1121-1128, (2003) Abstract | |
| Zhong, J., et al Nucleic Acids Res. 31th ed.,, 1656-1664, (2003) Abstract | |
| Jones, J.P., et al. Mol. Cancer Ther. 11, 687-694, (2005) |
